Tag: Mycobacterium tuberculosis

Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology 4.36/5 (70)

Colony Morphology on LJ Medium

Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation, a glycerated egg-based medium, is based upon Jensen’s modification. Jensen’s version eliminates congo […]

Acid-Fast Stain- Principle, Procedure, Interpretation and Examples 4.3/5 (438)

Interpretation of Acid-Fast Stain

Acid-Fast Stain- Principle, Procedure, Interpretation and Examples It is the differential staining techniques which was first developed by Ziehl and later on modified by Neelsen. So this method is also called Ziehl-Neelsen staining techniques. Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and counter stained with methylene blue. Thus […]

Habitat of Mycobacterium tuberculosis 3.77/5 (22)

Multidrug-resistant-Mycobacterium-tuberculosis

Habitat of Mycobacterium tuberculosis Patients with cavitary diseases are primary reservoirs. They are obligate pathogens, facultative or opportunistic pathogens or free living saprophytes. Most of them are found in habitats such as water or soil. It habitat human body from which is transmitted directly from person to person. Mycobacterium tuberculosis Complex contains tuberculosis, M. bovis, […]

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