Category: Staining Techniques

Negative Staining- Principle, Reagents, Procedure and Result 4.26/5 (104)

Result of Negative Staning

Negative Staining- Principle, Reagents, Procedure and Result The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed. It is also used to prepare biological samples for electron […]

Endospore Staining- Principle, Reagents, Procedure and Result 4.42/5 (60)

Procedure of Endospore Staining

Endospore Staining- Principle, Reagents, Procedure and Result In 1922, Dorner published a method for staining endospores. Shaeffer and Fulton modified Dorner’s method in 1933 to make the process faster The endospore stain is a differential stain which selectively stains bacterial endospores. The main purpose of endospore staining is to differentiate bacterial spores from other vegetative cells and […]

Capsule Staining- Principle, Reagents, Procedure and Result 4.27/5 (142)

Result of Capsule Staining

Capsule Staining- Principle, Reagents, Procedure and Result The main purpose of capsule stain is to distinguish capsular material from the bacterial cell. A capsule is a gelatinous outer layer secreted by bacterial cell and that surrounds and adheres to the cell wall. Most capsules are composed of polysaccharides, but some are composed of polypeptides. The capsule differs […]

Acid-Fast Stain- Principle, Procedure, Interpretation and Examples 4.3/5 (456)

Interpretation of Acid-Fast Stain

Acid-Fast Stain- Principle, Procedure, Interpretation and Examples It is the differential staining techniques which was first developed by Ziehl and later on modified by Neelsen. So this method is also called Ziehl-Neelsen staining techniques. Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and counter stained with methylene blue. Thus […]

Flagella – Introduction, Types, Examples, Parts, Functions and Flagella Staining- Principle, Procedure and Interpretation 4.3/5 (146)

Parts of Flagella

Flagella – Introduction, Types, Examples, Parts, Functions and Flagella Staining- Principle, Procedure and Interpretation Introduction of Flagella Flagella are the complex filamentous cytoplasmic structure protruding through cell wall. These are unbranched, long, thread like structures, mostly composed of the protein flagellin, intricately embedded in the cell envelope.  They are about 12-30 nm in diameter and […]

Gram Staining: Principle, Procedure, Interpretation, Examples and Animation 4.39/5 (265)

Gram Staining Interpretation

Gram Staining: Principle, Procedure, Interpretation, Examples and Animation Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms. Principle of […]

Microbiology Notes © 2014-2017            Site Created: 17th October, 2014            Copyright            Privacy Policy            Terms of Services            Contact Us Designed By: Sagar Aryal