Category: Biochemical Test

PYR Test- Principle, Uses, Procedure and Result Interpretation 4.83/5 (12)

Result Interpretation of PYR Test

PYR Test- Principle, Uses, Procedure and Result Interpretation PYR (Pyrrolidonyl Aminopeptidase) Test is used for the detection of pyrolidonyl arylamidase (also called pyrrolidonyl aminopeptidase) activity in Streptococcus pyogenes (group A strep), Enterococcus spp., some coagulase-negative staphylococci, and some Enterobacteriaceae. It is also known as PYR (L-pyrrolidonyl-β-naphthylamide) which serve as a substrate for the detection of […]

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation 4.89/5 (9)

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation Bile Solubility Test is the test which differentiate Streptococcus pneumoniae (positive- soluble) from alpha-hemolytic streptococci (negative- insoluble). Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are bile resistant. Principle of Bile Solubility Test S. pneumoniae has an autolytic enzyme which can be demonstrated by […]

Urease Test- Principle, Media, Procedure and Result 4.46/5 (65)

Result Interpretation of Urease Test

Urease Test- Principle, Media, Procedure and Result Urea Agar was developed by Christensen in 1946 for the differentiation of enteric bacilli. The urease test is used to determine the ability of an organism to split urea, through the production of the enzyme urease. Principle of Urease Test Urea is the product of decarboxylation of amino acids. Hydrolysis […]

Citrate Utilization Test- Principle, Media, Procedure and Result 4.52/5 (67)

Result Interpretation of Citrate Utilization Test

Citrate Utilization Test- Principle, Media, Procedure and Result This test is among a suite of IMViC Tests (Indole, Methyl-Red, Vogues-Proskauer, and Citrate) that are used to differentiate among the Gram-Negative bacilli in the family Enterobacteriaceae. Principle of Citrate Utilization Test Citrate agar is used to test an organism’s ability to utilize citrate as a source of energy. The […]

Voges–Proskauer (VP) Test- Principle, Reagents, Procedure and Result 4.41/5 (70)

Result Interpretation of Voges–Proskauer (VP) Test

Voges–Proskauer (VP) Test- Principle, Reagents, Procedure and Result Voges and Proskauer, in 1898, first observed the production of a red color after the addition of potassium hydroxide to cultures grown on specific media. Harden later revealed that the development of the red color was a result of acetyl-methyl carbinol production. In 1936 Barrit made the test […]

Methyl Red (MR) Test- Principle, Procedure and Result Interpretation 4.37/5 (65)

Result Interpretation of MR test

Methyl Red (MR) Test- Principle, Procedure and Result Interpretation The methyl red (MR) test detects the production of sufficient acid during the fermentation of glucose and the maintenance of conditions such that the pH of an old culture is sustained below a value of about 4.5, as shown by a change in the colour of […]

Indole Test- Principle, Reagents, Procedure, Result Interpretation and Limitations 4.57/5 (81)

Result Interpretation of Indole Test

Indole Test- Principle, Reagents, Procedure, Result Interpretation and Limitations This test demonstrate the ability of certain bacteria to decompose the amino acid tryptophane to indole, which accumulates in the medium. Indole production test is important in the identification of Enterobacteria. Most strains of E. coli, P. vulgaris, P. rettgeri, M. morgani and Providencia species break down the amino […]

Oxidase Test- Principle, Uses, Procedure, Types, Result Interpretation, Examples and Limitations 4.84/5 (93)

Dry Filter Paper Method

Oxidase Test- Principle, Uses, Procedure, Types, Result Interpretation, Examples and Limitations The oxidase test detects the presence of a cytochrome oxidase system that will catalyse the transport of electrons between electron donors in the bacteria and a redox dye- tetramethyl-p-phenylene-diamine. The dye is reduced to deep purple color. This test is used to assist in […]

Catalase Test- Principle, Uses, Procedure, Result Interpretation with Precautions 4.51/5 (129)

Result Interpretation of Catalase Test and Examples

Catalase Test- Principle, Uses, Procedure, Result Interpretation with Precautions This test demonstrate the presence of catalase, an enzyme that catalyses the release of oxygen from hydrogen peroxide (H2O2). It is used to differentiate those bacteria that produces an enzyme catalase, such as staphylococci, from non-catalase producing bacteria such as streptococci. Normally 3% H2O2 is used for the routine culture while […]

Coagulase Test- Principle, Procedure, Types, Interpretation and Examples 4.4/5 (92)

Coagulase Test- Principle, Procedure, Types, Interpretation and Examples Introduction to Coagulase Test Coagulase test is used to differentiate Staphylococcus aureus (positive) which produce the enzyme coagulase, from S. epidermis and S. saprophyticus (negative) which do not produce coagulase. i.e Coagulase Negative Staphylococcus (CONS). Principle of Coagulase Test Coagulase is an enzyme-like protein and causes plasma to clot by […]

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