Category: Bacterial Identification

Biochemical Test and Identification of Clostridium difficile 5/5 (9)

Biochemical Test and Identification of Clostridium difficile

Biochemical Test and Identification of Clostridium difficile ­Basic Characteristics Properties (Clostridium difficile) Capsule Capsulated Catalase Negative (-ve) Flagella Flagellated Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Motile Nitrate Reduction Negative (-ve) OF (Oxidative-Fermentative) Anaerobic Oxidase Negative (-ve) Shape Rods Spore Positive (+ve) Urease Negative […]

Biochemical Test and Identification of Streptococcus pyogenes 4.31/5 (13)

Biochemical Test and Identification of Streptococcus pyogenes

Biochemical Test and Identification of Streptococcus pyogenes ­Basic Characteristics Properties (Streptococcus pyogenes) CAMP Negative (-ve) Capsule Capsulated Catalase Negative (-ve) Gram Staining Gram Positive (+ve) Hemolysis Beta Hemolytic Motility Non-Motile OF (Oxidative-Fermentative) Facultative Anaerobes PYR Positive (+ve) Shape Cocci Spore Non-Sporing Urease Negative (-ve) VP (Voges Proskauer) Negative (-ve) 6.5% NaCl Growth Negative (-ve) Fermentation […]

Rapid Plasma Reagin (RPR) Test for the diagnosis of Syphilis 4.58/5 (12)

Rapid-Plasma-Reagin-(RPR)-Test-for-the-diagnosis-of-Syphilis

Rapid Plasma Reagin (RPR) Test for the diagnosis of Syphilis Introduction Rapid plasma reagin (RPR) is macroscopic, non treponemal, flocculation card test used to screen for syphilis caused by Treponema pallidum. RPR is simple test can be done within few minutes. This test is less sensitive than treponemal test in early syphilis infection. Two types […]

Biochemical Test and Identification of Staphylococcus epidermidis 4.81/5 (21)

Biochemical Test and Identification of Staphylococcus epidermidis

Biochemical Test and Identification of Staphylococcus epidermidis ­Basic Characteristics Properties (Staphylococcus epidermidis) Capsule Mostly Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Motility Negative (-ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Positive (+ve) Oxidase Negative […]

Biochemical Test and Identification of Bacillus anthracis 4.38/5 (21)

Biochemical Test and Identification of Bacillus anthracis

Biochemical Test and Identification of Bacillus anthracis ­Basic Characteristics Properties (Bacillus anthracis) Capsule Capsulated Catalase Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) Hemolysis Negative (-ve) Indole Positive (+ve) Motility Negative (-ve) Nitrate Reduction Positive (+ve) Oxidase Negative (-ve) Pigment Negative (-ve) Shape Rods Spore Positive (+ve) Urease Negative (-ve) […]

Biochemical Test and Identification of Bacillus cereus 4.27/5 (48)

Biochemical Test and Identification of Bacillus cereus

Biochemical Test and Identification of Bacillus cereus ­Basic Characteristics Properties (Bacillus cereus) Catalase Positive (+ve) Citrate Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) Growth in KCN Positive (+ve) Hemolysis Positive (+ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Variable Oxidase Negative (-ve) Pigment Negative (-ve) Shape […]

PYR Test- Principle, Uses, Procedure and Result Interpretation 4.85/5 (13)

Result Interpretation of PYR Test

PYR Test- Principle, Uses, Procedure and Result Interpretation PYR (Pyrrolidonyl Aminopeptidase) Test is used for the detection of pyrolidonyl arylamidase (also called pyrrolidonyl aminopeptidase) activity in Streptococcus pyogenes (group A strep), Enterococcus spp., some coagulase-negative staphylococci, and some Enterobacteriaceae. It is also known as PYR (L-pyrrolidonyl-β-naphthylamide) which serve as a substrate for the detection of […]

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation 4.89/5 (9)

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation

Bile Solubility Test- Principle, Reagents, Procedure and Result Interpretation Bile Solubility Test is the test which differentiate Streptococcus pneumoniae (positive- soluble) from alpha-hemolytic streptococci (negative- insoluble). Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are bile resistant. Principle of Bile Solubility Test S. pneumoniae has an autolytic enzyme which can be demonstrated by […]

CAMP Test- Principle, Uses, Procedure and Result Interpretation 4.82/5 (17)

CAMP Test- Principle, Uses, Procedure and Result Interpretation

CAMP Test- Principle, Uses, Procedure and Result Interpretation CAMP test is used to distinguish the species Streptococcus agalactiae from other species of beta-hemolytic Streptococcus. S. agalactiae, a member of the Lancefield Group B streptococci, is one of the causative agents of mastitis in cows. CAMP is an acronym for the authors of this test (Christie, […]

Biochemical Test and Identification of Salmonella Typhi 4.37/5 (189)

Biochemical Test and Identification of Salmonella Typhi

Biochemical Test and Identification of Salmonella Typhi Characteristics Salmonella Typhi Capsule Negative (-ve) Catalase Positive (+ve) Citrate Negative (-ve) Flagella Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Negative (-ve) H2S Positive (+ve) Indole Negative (-ve) Motility Motile MR (Methyl Red) Positive (+ve) MUG Test Negative (-ve) […]

Biochemical Test and Identification of Klebsiella pneumoniae 4.6/5 (122)

Biochemical Test and Identification of Klebsiella pneumoniae

Biochemical Test and Identification of Klebsiella pneumoniae Characteristics Klebsiella pneumoniae Capsule +ve Catalase +ve Citrate +ve Flagella -ve Gas +ve Gelatin Hydrolysis -ve Gram Staining -ve Growth in KCN +ve H2S -ve Indole -ve Motility -ve MR (Methyl Red) -ve MUG Test +ve Nitrate Reduction +ve OF (Oxidative-Fermentative) Fermentative Oxidase -ve Pigment -ve Shape Rod Spore […]

Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology 4.32/5 (66)

Colony Morphology on LJ Medium

Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation, a glycerated egg-based medium, is based upon Jensen’s modification. Jensen’s version eliminates congo […]

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