| Gram Staining | Negative |
| Shape (Cocci/Diplococci/Rods) | Rods |
| Motility (Motile / Non-Motile) | Motile |
| Capsule (Capsulated/Non-Capsulated) | Variable |
| Spore (Sporing/Non-Sporing) | Non-Sporing |
| Flagella (Flagellated/Non-Flagellated) | Flagellated |
| Catalase | Positive (+ve) |
| Oxidase | Negative (-ve) |
| MR | Positive (+ve) |
| VP | Negative (-ve) |
| OF (Oxidative/Fermentative) | Fermentative |
| Indole | Positive (+ve) |
| Citrate | Negative (-ve) |
| Urease | Negative (-ve) |
| Nitrate Reduction | Positive (+ve) |
| H2S | Negative (-ve) |
| Gas | Positive (+ve) |
| PYR | – |
| CAMP | – |
| Gelatin | Negative (-ve) |
| Niacin | – |
| Coagulase | – |
| Hemolysis (Alfa/Beta/Gamma) | Some Strains shows Hemolysis |
| Nagler | – |
| String Test | – |
| Pigment | – |
| Bile Solubility | – |
| Fermentation of | |
| Arabinose | Positive (+ve) |
| DNase | Negative (-ve) |
| Fructose | – |
| Glucose | Positive (+ve) |
| Hippurate | – |
| Inositol | Negative (+ve) |
| Inulin | – |
| Lactose | Positive (+ve) |
| Maltose | – |
| Mannitol | Positive (+ve) |
| Mannose | – |
| MyoInositol | – |
| Raffinose | – |
| Ribose | – |
| Sorbitol | Positive (+ve) |
| Sucrose | Variable |
| Enzymatic Reactions | |
| Acetate Utilization | Positive (+ve) |
| Acid Phosphatase | – |
| Alkaline Phosphatase | – |
| Amidase | – |
| Arginine dehydrolase | Variable |
| Arylsulphatase | – |
| Beta Lactamase | – |
| Hyalurodinase | – |
| Lecithinase | – |
| Lipase | – |
| Lysine | Positive (+ve) |
| Neuraminidase | – |
| ONPG Test | Positive (+ve) |
| Ornithine decarboxylase | Variable |
| Phenylalanine deaminase | Negative (-ve) |
| Perooxidase | – |
| Tellurite | – |
| Tributyrin | – |
| Differential Antibiotic | |
| Novobiocin | – |
| Bacitracin | – |
| Optochin | – |
| Streptomysin | – |
How to differentiate between motile and non motile strains of ecoli biochemically??
Is fermentation of fructose gram + or gram -?
How to identify for e.coli of MIC
How do we prepare biochemical sugars from solid state to solution state,and at what concentration are they used?
Mannitol is an alcohol-based compound, the mannitol solution does not contain salt in it. What you guys are arguing about was probably Mannitol salt agar.
for sodium carbonate should E coli be acidic or alkaline?
Can you please help me, with some test who detected the
1. Candida spp and
2. Gardnerella vaginalis?
in form +;- in who react and who nonreact. In the way of escherichia coli test.
Honestly,
Annais
Our lake has been tested for E. Coli and the results were given as such: 1: >80. 2: 5. 3: 5. and 4: 55. I assume they did 5 separate tests but did not explain if these are high or low and whether our is contaminated to the point that we cannot swim in or uuse its water to take showers or let the dogs in it since they drink the water. Can you help us figure this out.
Mnnitol culture E.coli may be a new strain of E.coli because Mannitol have 9-11% NACL and every type of bacteria could not tolrate such amount of salt concentration.
It need to identify on Molecular basis
Isn’t E.coli mannitol negative?
Some strains might be (not sure), but I plated it on mannitol salt agar last week and it was positive.