Biochemical Test and Identification of Clostridium difficile

Basic CharacteristicsProperties (Clostridium difficile)
CapsuleCapsulated
CatalaseNegative (-ve)
FlagellaFlagellated
Gelatin HydrolysisPositive (+ve)
Gram StainingPositive (+ve)
H2SPositive (+ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityMotile
Nitrate ReductionNegative (-ve)
OF (Oxidative-Fermentative)Anaerobic
OxidaseNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
Fermentation of
ArabinoseNegative (-ve)
CellobiosePositive (+ve)
DulcitolVariable
FructosePositive (+ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Enzymatic Reactions
Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LipaseNegative (-ve)
Tryptophan DeaminaseNegative (-ve)

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