Biochemical Test and Identification of Bacillus subtilis 4.4/5 (52)

Biochemical Test and Identification of Bacillus subtilis

Biochemical Test and Identification of Bacillus subtilis

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­Basic Characteristics

Properties (Bacillus subtilis)

Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Flagellated
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Variable
Pigment Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Variable
Glucose Positive (+ve)
Glycerol Positive (+ve)
Glycogen Positive (+ve)
Inositol Positive (+ve)
Inulin Variable
Lactose Variable
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Positive (+ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Positive (+ve)
Sorbitol Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Arginine Dehydrolase Negative (-ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)
Tyrosine Hydrolysis Negative (-ve)

Biochemical Test and Identification of Bacillus subtilis

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The Author

Sagar Aryal

I am Sagar Aryal, a passionate Microbiologist and the Scientific Blogger. I did my Master's Degree in Medical Microbiology and currently working as a Lecturer at Department of Microbiology, St. Xavier's College, Kathmandu, Nepal. I am particularly interested in research related to Medical Microbiology and Virology. Find me on Facebook, Twitter or Linkedin !!!

3 Comments

Add a Comment
  1. Thanks for the intel, But I would like to test my own Bacillus,
    Is there any protocol I can follow to test?

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